Subtipificación molecular de Salmonella entérica serotipo Enteritidis en el período post epidémico

Resumen

Background: In the last two decades, Salmonella enterica serotype
Enteritidis has become one of the main agents causing food borne diseases worldwide. This agent
is transmitted mainly by contaminated meat and poultry. Aim: To determine the genetic subtypes
of Salmonella enterica serotype Enteritidis, circulating in Chile between 2001 and 2003, a post
epidemc period. Material and methods: One hundred ninety three isolates coming from human
samples, prepared foods and animal products for human consumption, were analyzed by pulsed
field electrophoresis, using PulseNet standardized protocol. Results: Thirteen subtypes of
Salmonella enterica serotype Enteritidis were identified, that had between 0 and 13 bands. A
predominant subtype was identified in 172 strains (88%) that came from human isolates,
prepared foods and animal products for human consumption. Other four subtypes, found in
prepared foods and animal products for human consumption, were also found in human
isolates. Most subtypes were tightly interrelated Subtypes II, VIII and XI were also found in the
1994 epidemic. Conclusions: Subtyping of bacterial strains by pulsed field electrophoresis is
useful for the surveillance of food borne diseases